Anion Exchanger SAX W33 （Ascorbic Acid）

Anion Exchangers

UltraSep ES SAX W (Anorganic ions according to DIN 38405)

These anion exchangers are so called „permanently loaded“ ion exchangers. They exhibit the excellent performance of silicas and the good stability of RP-phases, which leads to low costs.

Stability of UltraSep ES SAXW with phthalic acid/TRIS buffer (1.25/2.5 mM) at pH 6.4

Permanently loaded ion exchangers are not only useful to separate inorganic ions (see Application ANION1) but also to separate natural products with aqueous buffers (nucleotides). They cannot be used with organic-aqueous eluents. 

Our permanently loaded ion exchangers are easily to regenerate. For this, we offer a special solution or a regeneration in our firm. 

If there is a need to chromatograph aqueous solutions highly contaminated with organic material, we recommend to use additionally a RP precolumn (length 33 mm) in front of the precolumn cartridge ( ANION2 Tap water from Berlin).

By lowering of the strength of the eluent, phosphate appears between nitrite and bromide without the necessity to increase the pH noticeably  In this way, there are good conditions for the conductivity detection too. Furthermore, fluoride is well separated from the injection peak (see Application ANION3).

UltraSep ES SAX W Quick Columns

See Applications ANION5 and ANION6

UltraSep ES SAX W33 (Determination of ascorbic acid)

See Applications ANION9

UltraSep ES SAX NN (Rapid determination of nitrite, nitrate, and bromide in drinking water and foodstuffs)

By this exchanger concentrations of nitrite and nitrate can be determined considerably faster and more sensitive than with customary methods. Nitrate, which can be determined by the photometric method only relatively insensitively, is determined by chromatography at 220 nm without previous enrichment to 0.1 mg/l. Bromide is determined at 210 nm with high sensitivity.

Iodide can be determined also directly at 225 nm. For a quick determination of iodide you can use a column of  UltraSep ES SAX NN with a length of 33 mm.

The selectivity and rapidity of the determination (phosphoric acid as eluent) can be changFor columns with endcapped packings an additional charge of 10.00 € is calculated.  ed by the pH value (at best pH 3 – 5). Furthermore,  the velocity of elution can also be varied by the concentration of phosphoric acid (at best 10 – 30 mM; see Application ANION4).

UltraSep ES RP NN (Determination of nitrite, nitrate, bromate and bromide in drinking water and foodstuffs)

Examples of Analyzed Compounds

Analyzed in Application
(AN = ANION)

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Ascorbic acid

AN9

Bromate

AN7, AN8

Bromide

AN1, AN3, AN4, AN5, AN6, AN7

Carbonate

AN2

Chloride

AN1, AN2, AN3, AN5, AN6

Fluoride

AN3

Nitrate

AN1, AN3, AN4, AN5, AN6, A7

Nitrite

AN1, AN3, AN4, AN5, AN6, A7

Phosphate

AN3, AN6

Sulfate

AN1, AN2, AN3, AN5, AN6

Size (mm)